NAPE Research Technologist

Job Description The Stuber Lab in the Center for the Study of the Neurobiology of Addiction, Pain and Emotion Research Lab (NAPE) has an outstanding opportunity for Research Technologist I to join their team. About this Opportunity Reporting to the Principle Investigator, the Research Technologist I is responsible for assisting with the study of elucidating the activity patterns of dopaminergic striatal neuronal circuits.

In this study, a variety of genetically modified mice are used. Optimizing and benchmarking biological sensors in vivo is needed.

Key Responsibilities

Assist on Research Projects- 75% Prepare shared stock solutions such as PBS and 4% paraformaldehyde. Operate histology experiment that includes mouse perfusion, brain sectioning, immunostaining, in situ hybridization, microscopy imaging, and analysis. Perform mouse behavioral studies and surgery under supervisor’s instruction.

Compile the data and present to supervisor and schedule next experiment. Mouse Colony Management- 20% Maintain compliance with UW animal care policies and monitor animal health, communicating with veterinary staff and taking appropriate action when concerns arise. Collaborate with the supervisor to develop and implement breeding strategies, including setting up breeding cages, monitoring mating success, and weaning pups.

Perform mouse genotyping procedures, including ear punching, DNA extraction, PCR, electrophoresis, and image acquisition. Maintain accurate colony records in mLIMS, manage cage inventory and housing needs, coordinate cage transfers between facilities, and provide regular project updates to the supervisor. Assist Lab Manager with Administrative Tasks - 5% Support daily laboratory operations and provide administrative assistance to the lab manager as needed.

Maintain laboratory equipment and shared resources, including dishwashing and autoclaving laboratory glassware. Monitor, inventory, and replenish laboratory supplies to ensure uninterrupted research operations. Assist with onboarding and training new undergraduate students and postdoctoral scholars, and support administrative processes such as submitting packing slips to the finance department.

Required Qualifications To be considered for this opportunity your application must demonstrate you meet both the minimum qualifications and additional qualifications listed below. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Minimum Qualifications

Applicants who do not meet these qualifications WILL NOT be forwarded to the Hiring Department. Two years experience as a Laboratory Technician II, or equivalent; OR a Bachelors Degree in an appropriate field of science or technology. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Additional Qualifications Experience with mouse care including handling, husbandry and familiar with animal use policies. Other procedures include administering euthanasia, performing perfusion, and sacrificing rodents.) Experience with mouse colony management including breeding and genotyping. Experience with histology experiment: Perfusion, sectioning with vibratome or cryostat, immunostaining or in situ hybridization, image acquisition, data analysis.

Experience with handling chemicals. Having strong PC skills like Microsoft Office applications. Excellent in communication and teamwork. Excellent in time-management and organizing skills.

Preferred Qualifications

Experience with a variety of scientific software's. For image analysis, image J, Olympus Cell. Sens, HALO and Zen. For data visualization, Corel. Draw, Adobe illustrator and photoshop. Experience with writing code with Python or R. Experience with animal surgery and behavior experiment.

About the Team

The Stuber lab is associated with the Departments of Anesthesiology and Pain Medicine, and Pharmacology, located at the University of Washington’s Health Science Building. In this highly collaborative environment, the research lab’s main goal is to delineate the neural circuits involved in the manifestation of neuropsychiatric disorders including addiction, maladaptive eating behaviors, and anxiety.

On a larger scale, the lab aims to determine how neural circuit elements directly regulate motivated behavioral states. In order to accomplish this, the lab uses in vivo fiber photometry, 2-photon imaging, optogenetic, and chemogenetic manipulations during measurements of naturalistic behavior. Compensation, Benefits and Position Details Pay Range Minimum: $45,144.

00 annual Pay Range Maximum: $53,148.00 annual Other Compensation:

• Benefits: For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-uw-staff/ Shift: First Shift (United States of America) Temporary or Regular? This is a regular position FTE (Full-Time Equivalent): 100.00% Union/Bargaining Unit: SEIU Local 925 Research Tech About the UW Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world. UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty. Our Commitment The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81 . To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [email protected] . Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law .